Identification of Barbiturates by Thin-layer Chromatography.

in cases of overdose and identification of the actual barbiturate concerned may be impossible because of the closely similar Rf values of this group. Jackson (1960) described treatment with hot sulphuric acid before paper chromatography to distinguish between barbiturates of similar chromatographic mobility. The inert nature of the silica gel supporting medium used in thin-layer separations enables this acid treatment to be carried out directly on the plate with consequent convenience and saving of time.